Biomedical optics

Nanostructures for bioimaging and theraphy

This research concerns two classes of innovative contrast agents: Inorganic nanoparticles which exhibit upconversion fluorescence (UCF; e.g., Yb3+,Tm3+:NaYF4) and nanostructures engineered from animal erythrocytes and containing IR-absorbing dye ICG. Such biocompatible nanoprobes have great potential for diagnostic imaging and theraeuptic applications.

Collaborations with Department for Materials Synthesis, IJS; Department for Inorganic Chemistry and Technology, IJS; University of California at Irvine; and University of California at Riverside, USA.

References:

PLOHL, Olivija, KRALJ, Slavko, MAJARON, Boris, FRÖHLICH, Eleonor, PONIKVAR-SVET, Maja, MAKOVEC, Darko, LISJAK, Darja. Amphiphilic coatings for the protection of upconverting nanoparticles against dissolution in aqueous media. Dalton transactions, 2017, vol. 46 (21), 6975-6984.

BURNS, Joshua M., JIA, Wangcun, NELSON, J. Stuart, MAJARON, Boris, ANVARI, Bahman. Photothermal treatment of port wine stains using erythrocyte-derived particles doped with indocyanine green: a theoretical study, Journal of Biomedical Optics, 2018, vol. 23 (12).

BURNS, Joshua M., SAAGER, Rolf, MAJARON, Boris, JIA, Wangcun, ANVARI, Bahman. Optical properties of biomimetic probes engineered from erythrocytes. Nanotechnology, 2017, vol. 28, no. 3, pp. 035101-1-035101-12.

LISJAK, Darja, PLOHL, Olivija, VIDMAR, Janja, MAJARON, Boris, PONIKVAR-SVET, Maja. Dissolution mechanism of upconverting AYF4:Yb,Tm (A = Na or K) nanoparticles in aqueous media. Langmuir, 2016, vol. 32, no. 32, str. 8222-8229.

LISJAK, Darja, PLOHL, Olivija, PONIKVAR-SVET, Maja, MAJARON, Boris. Dissolution of upconverting fluoride nanoparticles in aqueous suspensions. RSC advances, 2015, vol. 5, no. 35, str. 27393-27397.

Laser medicine and surgery

Our group is engaged in development of inovative laser treatments and improvement of existing irradiation protocols, primarily in the fields of dermatology (port wine stains), aesthetic surgery (skin rejuvenation, lipolysis), and dentistry. The approaches include detailed numerical modeling, testing in animals models, and clinical trials.

Collaboration with Fotona, d.o.o., Ljubljana, and University of California at Irvine, USA.

References:

MAJARON, Boris, MILANIČ, Matija, PREMRU, Jan. Monte Carlo simulation of radiation transport in human skin with rigorous treatment of curved tissue boundaries. Journal of biomedical optics, 2015, vol. 20, no. 1, str. 015002-1-015002-11.

MILANIČ, Matija, MAJARON, Boris. Energy deposition profile in human skin upon irradiation with a 1,342 nm Nd:YAP laser. Lasers in surgery and medicine, 2013, vol. 45, no. 1, str. 8-14.

JIA, Wangcun, TRAN, Nadia, SUN, Victor, MARINČEK, Marko, MAJARON, Boris, CHOI, Bernard, NELSON, J. Stuart. Photocoagulation of dermal blood vessels with multiple laser pulses in an in vivo microvascular model. Lasers in surgery and medicine, 2012, vol. 44, no. 2, str. 144-151.

MILANIČ, Matija, JIA, Wangcun, NELSON, J. Stuart, MAJARON, Boris. Numerical optimization of sequential cryogen spray cooling and laser irradiation for improved therapy of port wine stain. Lasers in surgery and medicine, 2011, vol. 43, no. 2, str. 164-175.

MILANIČ, Matija, MAJARON, Boris. Three-dimensional Monte Carlo model of pulsed-laser treatment of cutaneous vascular lesions. Journal of biomedical optics, 2011, vol. 16, no. 12, str. 128002-1-128002-12.

Noninvasive characterization of human tissue in vivo

We are developing inovative approaches for noninvasive characterization of biological tissues in vivo, primarily involving pulsed photo-thermal radiometry and/or diffuse reflectance spectroscopy. Potential practical applications of current interest are objective analysis of dermatologic laser treatments (e.g., skin rejuvenation, removal of tatoos) and assessment of the age of traumatic bruises (for forensic investigations).

Collaboration with Fotona d.o.o., Ljubljana, and Department of knowledge technologies (E8).

References:

VERDEL, Nina, LENTSCH, Griffin, BALU, Mihaela, TROMBERG, Bruce J., MAJARON, Boris. Noninvasive assessment of skin structure by combined photothermal radiometry and optical spectroscopy : coregistration with multiphoton microscopy. Applied optics, 2018, vol. 57, no. 18, str. D117-D122.

VIDOVIČ, Luka, MILANIČ, Matija, MAJARON, Boris. Objective characterization of bruise evolution using photothermal depth profiling and Monte Carlo modeling. Journal of biomedical optics, vol. 20, no. 1, str. 017001-1-12.

VIDOVIČ, Luka, MAJARON, Boris. Elimination of single-beam substitution error in diffuse reflectance measurements using an integrating sphere. Journal of biomedical optics, 2014, vol. 19, no. 2, str. 027006-1-027006-9.

VIDOVIČ, Luka, MILANIČ, Matija, MAJARON, Boris. Influence of the sampling rate and noise characteristics on prediction of the maximal safe laser exposure in human skin using pulsed photothermal radiometry. International journal of thermophysics, 2013, vol. 34 (8/9), 1549-1558.

MILANIČ, Matija, MAJARON, Boris. Characterization of laser tattoo removal treatment using pulsed photothermal radiometry. LAHA: Journal of the laser and health academy, 2013, vol. 2013 (1), 68-74.